Welcome to the METABARPARK Project website. The project aims to apply the new high throughput sequencing technologies to characterize benthic communities in the Spanish Marine National Parks using environmental DNA (metabarcoding). Our main objective is to characterize the hidden biodiversity which is usually overlooked by traditional methods. We will also try to assess the community impacts of invasive seaweeds.

Monday, June 15, 2015

Working with the second gene

In March we received the results of the Illumina run performed on our samples amplified for the COI gene. Unfortunately the quality of the reads was no good enough and we had to discard many of them. This led us to ask for a repetition of the run with a new protocol for preparation of the genetic libraries. Fasteris was up to the challenge and repeated the run for free. The results of the new run arrived in May, and this time most of the reads could be used.

With another 14 milion sequences to work with, we are still adjusting our pipelines to meet the specificities of COI. We had to construct a new reference database using the Barcode Of Life Data (BOLD) systems. We hope to have results of this second gene in short!
Posted by at

No comments:

Post a Comment

Subscribe to: Post Comments (Atom)