Processing samples

We are now in the midle of sample processing. The work consists of separating the samples into three size fractions using sieves of 10, 1, and 0.06 mm. In this way each sample becomes three according to the organisms' size. Afterwards the DNA is extracted separately for each fraction using a special kit (PowerMax Soil Extraction Kit). The DNA is later amplified using universal primers for the target gene (we will begin using 18S).
As of today, the samples from one of the trips have been already sorted and we start the extractions... coming closer!

Sorting samples with a column of sieves

The three size fractions of a sample